Invention for Method of delivering molecules lyophilized on microparticles into plant tissues

Invented by Susana Martin-Ortigosa, Kan Wang, Iowa State University Research Foundation ISURF

The delivery of molecules into plant tissues is a crucial aspect of modern agriculture and biotechnology. It is essential for the development of new crop varieties, the production of high-value plant-based products, and the improvement of plant health and resistance to pests and diseases. However, the delivery of molecules into plant tissues is a challenging task, as plant cells have a complex and rigid cell wall that restricts the entry of foreign molecules.

One promising approach to overcome this challenge is the use of microparticles as a delivery vehicle. Microparticles are tiny particles that can be loaded with molecules and delivered into plant tissues. They can protect the molecules from degradation and facilitate their uptake by plant cells. Moreover, microparticles can be designed to release the molecules in a controlled manner, providing a sustained and localized delivery.

One of the most promising methods for delivering molecules lyophilized on microparticles into plant tissues is freeze-drying, also known as lyophilization. Freeze-drying is a process that removes water from a sample by sublimation, without melting it. This process can preserve the integrity of the molecules and the microparticles, allowing them to be stored and transported at room temperature for extended periods.

The market for the method of delivering molecules lyophilized on microparticles into plant tissues is growing rapidly, driven by the increasing demand for sustainable and efficient agricultural practices. The use of microparticles for delivering molecules can reduce the amount of chemicals needed for crop protection and improve the efficiency of nutrient delivery. Moreover, it can enable the production of high-value plant-based products, such as pharmaceuticals, nutraceuticals, and cosmetics.

Several companies are currently developing and commercializing products based on the delivery of molecules lyophilized on microparticles into plant tissues. For example, Vestaron Corporation is using this approach to develop a new class of biopesticides that can target specific pests while minimizing the impact on non-target organisms. AgroSpheres is developing a platform for the delivery of crop protection agents using biodegradable microparticles. And Indigo Agriculture is using microparticles to deliver beneficial microbes to plant roots, improving nutrient uptake and stress tolerance.

In conclusion, the market for the method of delivering molecules lyophilized on microparticles into plant tissues is a promising and rapidly growing field. This approach has the potential to revolutionize agriculture and biotechnology by enabling the efficient and sustainable delivery of molecules into plant tissues. As the demand for sustainable and efficient agricultural practices continues to grow, the use of microparticles for delivering molecules is likely to become an essential tool for the development of new crop varieties, the production of high-value plant-based products, and the improvement of plant health and resilience.

The Iowa State University Research Foundation ISURF invention works as follows

The invention includes particles and methods for delivering freeze- or air dried molecules to cells.

Background for Method of delivering molecules lyophilized on microparticles into plant tissues

Definitions

Example Molecules & Compositions for Deliveries

Example I

Example II

Methods

Plant Materials

Microparticle-Molecule Lyophilization

Biolistic Method

X-Gluc Histochemical staining

Fluorescein Diacetate

Microscopy

Results

Co-delivery of Plasmid DNA with Protein

Discussion

Example III

Delivery Of Proteins Air Dried Onto Gold Projectiles

Example IV

Delivery Of Active?-Glucuronidase To Mouse Ear Pine Tissue Cells

Example V

Intracellular delivery of active?-glucuronidase to plant tissues air dried onto tungsten particles

Example VI

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