Invented by Inbal Nurith Dangoor, Hagai Karchi, Evogene Ltd
Introduction
Abiotic stress, such as drought, salinity, extreme temperatures, and nutrient deficiency, poses a significant threat to global agriculture. These stressors can severely impact crop growth, development, and ultimately, yield. To combat these challenges, scientists have been exploring various methods to enhance abiotic stress resistance, biomass production, and crop yield. One promising approach involves the use of isolated polynucleotides and polypeptides, along with innovative methods for their application.
Isolated Polynucleotides and Polypeptides
Isolated polynucleotides and polypeptides are molecules that have been extracted and purified from their natural sources. These molecules play crucial roles in regulating gene expression and protein synthesis, making them valuable tools for enhancing plant traits. In the context of abiotic stress resistance, isolated polynucleotides and polypeptides can be used to modulate specific genes and proteins involved in stress response pathways.
Methods for Using Isolated Polynucleotides and Polypeptides
Several methods have been developed to utilize isolated polynucleotides and polypeptides for increasing abiotic stress resistance, biomass, and crop yield. One common approach is genetic engineering, where specific genes or gene sequences are introduced into plants to enhance desired traits. This technique allows for the precise manipulation of plant genomes, enabling the introduction of stress-responsive genes or genes involved in biomass production.
Another method involves the use of biostimulants, which are substances that enhance plant growth and development. Isolated polynucleotides and polypeptides can be formulated into biostimulant products and applied to crops to improve their tolerance to abiotic stress and promote biomass accumulation. These biostimulants can be applied through foliar sprays, seed treatments, or soil applications, depending on the target crop and stress conditions.
Benefits and Market Potential
The market for isolated polynucleotides and polypeptides, along with the methods for their application, is rapidly expanding. The demand for crops with enhanced abiotic stress resistance and increased biomass is growing due to the increasing global population and the need for sustainable agriculture. Farmers and agricultural companies are seeking innovative solutions to mitigate the negative impacts of abiotic stress on crop productivity.
The use of isolated polynucleotides and polypeptides offers several benefits. Firstly, it allows for the development of stress-tolerant crops that can thrive under adverse environmental conditions, reducing yield losses. Secondly, it enables the production of crops with higher biomass, which can contribute to increased food, feed, and bioenergy production. Lastly, the use of these molecules can potentially reduce the reliance on chemical inputs, such as fertilizers and pesticides, leading to more sustainable agricultural practices.
Conclusion
The market for isolated polynucleotides and polypeptides, along with the methods for their application, holds immense potential for improving abiotic stress resistance, biomass production, and crop yield. As the demand for stress-tolerant crops and sustainable agricultural practices continues to rise, the use of these molecules will play a crucial role in meeting these challenges. Continued research and development in this field will pave the way for innovative solutions to enhance crop productivity and ensure global food security in the face of abiotic stress.
The Evogene Ltd invention works as follows
The invention provides isolated polypeptides that are at least 80 percent identical to SEQ NOs 529, 475-528 530-770 6179-9796 9798-1042, isolated polynucleotides that are at most 80 percent identical to SEQ NOs 314, 1-313 315-474 771-6178 and nucleic acids constructs containing same. It also includes transgenic cells and plants expressing the same.
Background for Isolated Polynucleotides and polypeptides, and methods for using the same to increase abiotic stress resistance, biomass, and yield of plants.
According to some embodiments, there is a method for increasing yield, growth, biomass and vigor of a plant. This includes expressing in the plant a exogenous nucleotide containing a sequence of nucleic acids encoding polypeptides at least 80% similar to SEQ ID No: 475-770. 6179-9796. 9798-10420. or 10421.
According to some embodiments, there is a method for increasing yields, growth rates, biomass and vigor of plants, as well as oil content. This can be achieved by expressing in the plant an exogenous nucleotide containing a sequence of nucleic acids encoding polypeptides from the SEQ ID Nos. 475-770, 6179-10421 and other similar numbers.
According to some embodiments, there is a method for producing a plant that includes growing a plant transformed with a exogenous nucleic-acid sequence encoding an amino acid sequence at least 80% similar to the sequence, the plant being derived from plants chosen for their increased yields, growth rates, biomass, vigor and oil content. The plant also has an increased seed yield and fiber yield as well as increased fiber quality and fiber length. It is also more resistant to abiotic stresses and increased photosy
According to some embodiments, there is a method for increasing yield, growth, biomass and vigor of a plant. This includes expressing in the plant a polynucleotide containing a nucleic sequence that is at least 80% similar to SEQ ID No: 1-474 or 771-6177.
According to some embodiments, there is a method for increasing yields, growth rates, biomass and vigor of plants, as well as their oil content and seed yields, fiber yields, fiber quality and fiber length. This involves expressing in the plant a polynucleotide containing the nucleic acids sequences selected from SEQ ID Nos:1-474, and 771-6178.
According to some embodiments, there is a method for producing a plant that involves growing a plant transformed with a polynucleotide that contains a nucleic sequence at least 80% similar to a sequence chosen from the group consisting SEQ ID Nos:1-474, 771-6178. The crop plant is then derived from plants which are selected for their increased yields, growth rates, biomass, oil content and seed yields, as well as increased fiber yields, fiber quality and fiber length.
According to some embodiments, there is a polynucleotide that is a nucleic sequence encoding polypeptide that contains an amino-acid sequence at least 80% similar to the amino-acid sequence in SEQ ID No:475-770. 6179-9796, 9798-10420, or 10421. This amino acid is capable of increasing the yield, growth rate and biomass of plants, as well as their vigor.
According to some embodiments of this invention, a polynucleotide is provided that contains a nucleic sequence encoding the polypeptide that includes the amino acid sequence chosen from the group consisting SEQ ID Nos: 475-770 and 6179-10421.
According to some embodiments, there is a polynucleotide that is at least 80% identical with SEQ ID Nos. 1-474 and 771-6878, which is capable of increasing the yield, growth rate and biomass of a plant, as well as its vigor and oil content. It can also increase seed yield and fiber yield and fiber quality and length.
According to some embodiments of this invention, a polynucleotide isolated containing the nucleic acids sequence is provided. The group of SEQ ID Nos: 1-474, 771-6178 and SEQ ID no. 1-474 are all included.
According to some embodiments, a nucleic-acid construct is provided that contains the isolated polynucleotide from some embodiments and a promoter used to direct transcription of the nucleic-acid sequence in the host cell.
According to some embodiments of this invention, an isolated polypeptide containing an amino acid peptide at least 80% similar to SEQ ID No: 475-770 or 6179-9796 is provided, wherein the amino acid peptide is capable of increasing yields, growth rates, biomass, vigor and oil content of plants.
According to some embodiments of this invention, a polypeptide is isolated that contains the amino acid sequence chosen from the group consisting SEQ ID Nos: 475-770 and 6179-10421.
According to some embodiments, a plant cell is provided that exogenously expresses the polynucleotide or nucleic acid of certain embodiments.
According to some embodiments, a plant cell expressing exogenously the polypeptide in some embodiments is provided.
According to certain embodiments of the present invention, the nucleic acids sequence encodes a sequence of amino acids selected from the group consisting SEQ ID Nos: 475-770 and 6179-10421.
According to certain embodiments of the present invention, the nucleic acids sequence is chosen from the following group: SEQ ID Nos. 1-474 and 771-6178.
According to certain embodiments of the present invention, polynucleotide is composed of a nucleic sequence chosen from the following SEQ ID Nos: 1-474 or 771-6178.
According to certain embodiments of the present invention, the nucleic acids sequence encodes an amino acid sequence chosen from the group consisting SEQ ID Nos: 475-770 and 6179-10421.
According to certain embodiments of the present invention, a plant cell is a part of a living plant.
According to certain embodiments of the present invention, the method also comprises growing the plant that expresses said exogenous nucleotide in the presence of abiotic stress.
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